Sheep anti BrdU (bromodeoxyuridine)

Catalogue Number: A205P-EXA

Manufacturer:	ExAlpha
Type:	Polyclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	250 ug
Host name:	Sheep
Clone:
Isotype:	IgG
Immunogen:
Application:	FC, ICC, IHC-P, IP, WB, IHC-Fr

Overview

Description

Description: Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining.

Additional Text

Purification

Ammonium Sulfate Precipitated

Antibody Clonality

Polyclonal

Application Notes

Applications: Immunoprecipitation: 25-100 µg/ml; Immunohistochemistry: 25-100 µg/ml. The antibody has been tested using immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 ug/ml, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100µg/ml. Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody. Applicable in flow cytometry for the quantification of Brdu labeled cells, indication the proliferative activity of the tested cell population.

Storage Note

Product should be stored at -20C. Aliquot to avoid freeze/thaw cycles

Price

£396.00

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