ATM, phosphorylated (Ser1981) (Ataxia Telangiectasia Mutated, ATA, AT Complementation Group A, ATC, ATD, ATE) (MaxLight 550)

Catalogue Number: A4000-26-ML550-USB

Manufacturer:	United States Biological
Physical state:	Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Type:	Monoclonal Primary Antibody - Conjugated
Alias:	AT mutated\|A-T mutated\|AT1\|ATA\|ataxia telangiectasia mutated\|ATC\|ATD\|ATDC\|ATE\|serine-protein kinase ATM\|TEL1\|TEL1, telomere maintenance 1, homolog\|TELO1
Shipping Condition:	Blue Ice
Unit(s):	100 ul
Host name:	Mouse
Clone:	5H48 (10H11.E12)
Isotype:	IgG1, kappa
Immunogen:	Synthetic peptide corresponding to aa1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM (KLH). The immunizing sequence has 11/12 identical amino acids in mouse and rat.
Application:	ICC, IP, WB

Overview
Datasheets

Description

Description: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1. The essential requirement for the substrates of ATM/ATR is S/TQ. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S/TQ are negative determinants for substrate phosphorylation. The complex phenotype of cells derived from patients with AT suggests that ATM has additional cellular substrates. In unirradiated cells, ATM is present as an inactive homodimer or multimer. Double-stranded breaks in DNA caused by ionizing radiation cause rapid ATM kinase activation through dissociation of this complex and ATM autophosphorylation at Ser1981.

Additional Text

Gene Name

ATM

Gene ID

472

Uniprot ID

Q13315

Accession Number

NP_000042

Specificity

Recognizes human ATM, Mr ~370kD when phosphorylated at Ser1981. A nonspecific protein was also detected, Mr ~> 400kD. Species Crossreactivity: mouse. Sequence Homology: rat

Purification

Protein G purified

Modification

Phosphorylated, Ser1981

Antibody Clonality

Monoclonal

A4000-26-ML550 Datasheet

Price

£979.00

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