Antigen Retrieval Buffer (100x EDTA, pH 8.0)
Catalogue Number: A456-LET
| Manufacturer: | Leinco Technologies, Inc |
| Type: | Histology Buffer |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 125 ml, 250 ml, 50 ml |
| Application: | IHC |
Description
Description: Immunohistochemistry relies on fixation to preserve tissues, but these treatments can have an unintended consequence: they mask the very proteins researchers are interested in. This masking occurs because fixation create cross-links between proteins, obscuring the specific sites (antigens) that antibodies need to bind to. Antigen retrieval buffer is designed for a technique called heat-induced epitope retrieval (HIER). HIER uses heat to break these protein cross-links, essentially "unmasking" the hidden antigen sites. This allows antibodies to bind more effectively, leading to clearer and more accurate results in immunohistochemistry experiments.
Additional Text
Storage Note
This buffer solution should be stored between 2°C and 8°C.
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