Anti-Human/Mouse/Rat Alpha Smooth Muscle (αSM) Actin (1A4) - Purified No Carrier Protein

Catalogue Number: A460-LET

Manufacturer:	Leinco Technologies, Inc
Type:	Monoclonal Primary Antibody - Unconjugated
Shipping Condition:	Blue Ice
Unit(s):	10 mg, 2 mg, 200 ug, 25 ug
Host name:
Clone:	1A4
Isotype:
Immunogen:
Application:	FC, IF, IHC-P, IP, WB, IHC, FA

Overview

Description

Description: Myofibroblasts are specialized connective tissue fibroblasts that use actin-based motors to generate contractile forces that are applied to extracellular matrix adhesion receptors 1. Alpha-smooth muscle actin (SMA) is a tissue specific actin isoform involved in myofibroblast differentiation, focal adhesion maturation, actin polymerization, tractional remodeling of the extracellular matrix, and the generation and transmission of mechanical forces in connective tissue, i.e., mechanotransduction. These physical forces are important for wound healing, joint and ligament remodeling due to injury or pregnancy, and the maintenance of tissue structure in the urinary bladder. SMA facilitates the generation of contractile forces in myofibroblasts and may contribute to scar formation and fibrocontractive diseases 1. Additionally, cancer-associated fibroblasts (CAF) play key roles in the tumor microenvironment, affecting tumor development, metabolism, and migration 2. SMA is highly expressed in CAFs and is widely used as a marker to identify CAF populations. However, SMA cannot be used for flow-sorting CAF populations for further functional studies because of its intracellular localization. 1A4 was produced using a synthetic decapeptide as immunogen, consisting of the NH2-terminal peptide of SMA coupled to BSA and a keyhole limpet hemocyanin (Ac-Glu(OBut)-Glu(OBut)-Glu(OBut)-Asp(OBut)-Ser(But)-Thr(But)-Ala-Leu-Val-Cys(Acm)-NHEt) 3. BALB/c mice were immunized and spleen cells were fused with Sp2/0 myeloma cells. Hybridomas were screened by ELISA against bovine aortic actin and further tested by immunofluorescence in chicken gizzard.

Additional Text

Purification

Purified

Antibody Clonality

Monoclonal

Concentration

1 mg/ml

Storage Note

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Price

£2,254.00

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