Sulfo-NHS-Biotin
Catalogue Number: A8001-APE
| Manufacturer: | Apexbio |
| Physical state: | A solid |
| Type: | Conjugation Reagent |
| Alias: | Sulfo-NHS Biotin |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg |
Description
Description: Sulfo-NHS-Biotin (N-Hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. The most common amine-reactive biotinylation reagents can be divied into two basic types, N-hydroxysuccinimide (NHS) esters and carboxylates. Generally, NHS esters biotinylation reagent contains a reactive NHS ring structure that reacts with an amine on the carbonyl group of a protein or other molecules through nucleophilic attack subsequently forming a stable amide linkage and releasing the NHS group. Due to the insolubility of NHS-biotin in aqueous environments, sulfo-NHS-biotin, a water-soluble analog of NHS-biotin, has been developed by attaching a negatively charged sulfonate group on the NHS ring structure, which can be added directly to aqueous reactions without the need for organic solvent dissolution.ReferenceBioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
Additional Text
Short Description
Amine-reactive biotinyltation reagent, water soluble
Reagent:Smile String
C1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
Solubility
≥22.17 mg/mL in DMSO; insoluble in EtOH; ≥59.9 mg/mL in H2O with ultrasonic
Storage Note
Desiccate at -20°CThe product is not stable in solution, please dissolve it immediately before use.
A8001 Datasheet
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