NHS-Biotin
Catalogue Number: A8002-APE
| Manufacturer: | Apexbio |
| Physical state: | A solid |
| Type: | Conjugation Reagent |
| Alias: | Biotin N-hydroxysuccinimide ester, Biotin-OSU, Biotin NHS, Biotin SE |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg |
Description
Description: NHS-Biotin (C14H18O5N3S) is N-hydroxysuccinimido biotin. It is the shortest of three similar EZ-Link NHS-Biotin Reagents that enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. NHS-Biotin offers researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Because it is uncharged and contain simple alkyl-chain spacer arms, NHS-Biotin compound is membrane-permeable and useful for intracellular labeling.NHS-Biotin is the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide. Ref:Fouassier, L., Yun, C. C., Fitz, J. G. and Doctor, R. B. (2000). Evidence for Ezrin-Radixin-Moesin-binding Phosphoprotein 50 (EBP50) Self-association through PDZ-PDZ Interactions. J. Biol. Chem. 275, 25039-25045.Nunomura, W., Takakuwa, Y., Parra, M., Conboy, J.G., & Mohandas, N. (2000) Regulation of protein 4.1R, p55 and glycophorin C ternary complex in human erythrocyte membrane. J. Biol. Chem. 275(32): 24540-24546.Chiu, N.H. & T.K. Christopoulos. 1999. Two-site expression immunoassay using a firefly luciferase-coding DNA label. Clin. Chem. 45: 1954-1959.
Additional Text
Short Description
Amine-reactive biotinyltation reagent
Reagent:Smile String
C1CC(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3
Solubility
insoluble in EtOH; insoluble in H2O; ≥17.07 mg/mL in DMSO
Storage Note
Desiccate at -20°C
A8002 Datasheet
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