NHS-LC-Biotin
Catalogue Number: A8004-APE
| Manufacturer: | Apexbio |
| Type: | Conjugation Reagent |
| Alias: | Succinimidyl 6-(biotinamido)hexanoate |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg |
Description
Description: NHS-LC-biotin (succinimidyl-6-(biotinamindo)hexanoate), also known as NHS-X-biotin is a derivative of D-biotin, a amine-reactive biotinylation agent, that contains a spacer arm off the valeric acid side chain of D-biotin with an NHS ester group at its end. The NHS ester group at the end of NHS-LC-biotin covalently binds to amine groups in proteins and other molecules forming a stable amide linkage and releasing the NHS group. The 6-aminocaproic acid spacer of NHS-LC-biotin greatly increases the length between a covalently modified molecule and the bicyclic biotin rings leading to a better binding potential for avidin or streptavidin probes. NHS-LC-biotin is insoluble in aqueous environments requiring the dissolution of organic solvents prior to the addition to a buffered reaction.ReferenceBioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
Additional Text
Storage Note
Store at -20°C
Short Description
Amine-reactive biotinylation reagent, long chain
Reagent:Smile String
C1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3
Solubility
≥22.73 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O
A8004 Datasheet
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