Catalogue Number: A8006-APE
Manufacturer: | Apexbio |
Type: | Conjugation Reagent |
Alias: | NHS-SS-Biotin,Biotin disulfide N-hydroxysuccinimide ester |
Shipping Condition: | Blue Ice |
Unit(s): | 1 g, 100 mg |
Description: NHS-SS-biotin (Succinimidyl-2-(biotinamido)-ethyl-1,3-dithiopropionate) is a long-chain cleavable amine-reactive biotinylation reagent that has a spacer arm of 24.3 A in length due to the existence of a cross-bridge within its chemical structure. The long spacer arm creates an increased length between a covalently modified molecule and the bicyclic biotin rings that greatly enhances the ability of the molecule to bind to avidin or streptavidin probes. The NHS ester group of NHS-SS-biotin reacts with the amine groups of proteins and other molecule forming an amide bond and releasing of sulfo-NHS. NHS-SS-Biotin is a water-insoluble molecule that requires the dissolution of organic solvents prior to be added to aqueous reactions.ReferenceBioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
Amine-reactive biotinylation reagent, mid-length
≥28.79 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH
Store at -20°CThe product is not stable in solution, please dissolve it immediately before use.
O=C1CCC(N1OC(CCSSCCNC(CCCC[C@@H]2SC[C@@](N3)([H])[C@]2([H])NC3=O)=O)=O)=O