Cy3 azide (non-sulfonated)

Catalogue Number: A8111-APE

Manufacturer:	Apexbio
Shelf Life:	24 months
Physical state:	A solid
Type:	Click Chemistry Reagent
Shipping Condition:	Blue Ice
Unit(s):	25 mg, 5 mg

Overview
Datasheets

Description

Description: Cy3 azide is a non-sulfonated dye ready for the use in Click Chemistry reaction which can be detected by various fluorometers, imagers, and microscopes. The reagent can be detected by naked eye on gels because of inherently high extinction coefficient. For biomolecule labeling, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. Product is available both as solid compound, and as 10 mM solution in DMSO. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. Water-soluble version of this reagent is also available. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. In protein analysis, proteins labeled by Cy3 azide couple with Cy5 azide can substantially simplifies the target identification process than only one fluorescent dye [1]. Reference:[1]. Park, H.; Koo, J.Y.; Srikanth, Y.V.V.; Oh, S.; Lee, J.; Park, J.; Park, S.B. Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells. Chemical Communications, 2016, 52(34), 5828-5831.

Additional Text

Short Description

used for Click chemistry to label alkyne-modified oligonucleotides

Reagent:Smile String

CC1(C)C2=CC=CC=C2[N+](CCCCCC(NCCCN=[N+]=[N-])=O)=C1/C=C/C=C3C(C)(C)C4=CC=CC=C4N3C

Solubility

≥28.75 mg/mL in DMSO; ≥14.77 mg/mL in H2O with ultrasonic; ≥77.2 mg/mL in EtOH with ultrasonic

Storage Note

24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Price

£1,080.00

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