Cy3 carboxylic acid (non-sulfonated)
Catalogue Number: A8132-APE
| Manufacturer: | Apexbio |
| Shelf Life: | 24 months |
| Physical state: | A solid |
| Type: | Fluorescent Reagents |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 mg, 5 mg |
Description
Description: Cy3 carboxylic acid is a non-activated fluorescence dye. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. This Non-sulfonated reagent has limited aqueous solubility, but with good solubility in organic solvents. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. In the supramolecular glutamine assay, Cy3 carboxylic acid was conjugated to a single strain DNA and then immobilized onto the glass slide through DNA hybridization. The Cy3 groups serve as FRET donor and the cyclodextrins as supramolecular host [1]. Reference:[1] Xue, M.; Wei, W.; Su, Y.; Johnson, D.; Heath, J.R. Supramolecular Probes for Assessing Glutamine Uptake Enable Semi-Quantitative Metabolic Models in Single Cells. Journal of the American Chemical Society, 2016, 138(9), 3085-3093.
Additional Text
Short Description
Non-reactive fluorophore, for control experiments, and for calibration
Reagent:Smile String
CC(C1=C(N/2C)C=CC=C1)(C)C2=C/C=C/C3=[N+](CCCCCC(O)=O)C(C=CC=C4)=C4C3(C)C.[Cl-]
Solubility
≥49.3 mg/mL in DMSO; insoluble in H2O; ≥99 mg/mL in EtOH
Storage Note
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
A8132 Datasheet
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