Cy5 carboxylic acid (non-sulfonated)
Catalogue Number: A8133-APE
| Manufacturer: | Apexbio |
| Shelf Life: | 24 months |
| Physical state: | A solid |
| Type: | Fluorescent Reagents |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 mg, 5 mg |
Description
Description: Cy5 carboxylic acid is a non-activated carboxylic acid with low aqueous solubility. For coupling with amines, or protein labeling, Cy5 NHS ester and water-soluble sulfo-Cy5 NHS ester is preferred. This labeling dye has limited water solubility. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. In the cells, The Cy5 carboxylic acid label on the dye can track the efficiency of the protein expression [1].Reference:[1] Deyle, K.M.; Farrow, B.; Hee, Y.Q.; Work, J.; Wong, M.; Lai, B.; Umeda, A.; Millward, S.W.; Nag, A.; Das, S.; Heath, J.R. A protein-targeting strategy used to develop a selective inhibitor of the E17K point mutation in the PH domain of Akt1. Nature Chemistry,2015, 7, 455-462.
Additional Text
Short Description
Non-reactive fluorophore, for control experiments, and for calibration
Reagent:Smile String
CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)O)(C)C)C)C
Solubility
≥51.9 mg/mL in DMSO; insoluble in H2O; ≥89 mg/mL in EtOH
Storage Note
24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
A8133 Datasheet
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