Cy5 maleimide (non-sulfonated)

Catalogue Number: A8139-APE

Manufacturer:	Apexbio
Shelf Life:	24 months
Physical state:	A solid
Type:	Fluorescent Reagents
Shipping Condition:	Blue Ice
Unit(s):	25 mg, 5 mg

Overview
Datasheets

Description

Description: Cy5 maleimide is a mono-reactive dye for selectively labeling peptides and proteins which couples with thiol groups. The labeling reagent is a common fluorophore dye with low aqueous solubility which is compatible with various instrumentation like microscopes, imagers, and fluorescence readers. Cy5 maleimide is part of the probe among the biological research. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer. The user can generate probes with virtually any non-genetically encoded moiety of interest with the scaffold in hand.G3C(Cy5) probe consists of two parts. First, a GGGC scaffold peptide is synthesized on solid phase, followed by coupling of the liberated peptide to Cy5 maleimide, while in solution [1].Reference:[1]. Popp, M.W. Site-Specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist. Methods in Molecular Biology,2015, 1266, 185-198.

Additional Text

Short Description

Labeling of cysteine residues, as well as other thiolated molecules

Reagent:Smile String

CC1(C2=CC=CC=C2[N+](=C1C=CC=CC=C3C(C4=CC=CC=C4N3CCCCCC(=O)NCCN5C(=O)C=CC5=O)(C)C)C)C

Solubility

≥64.1 mg/mL in DMSO; insoluble in H2O; ≥65 mg/mL in EtOH

Storage Note

24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Price

£328.00

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