Anti-Fluorescein [4-4-20 (enhanced)]

Catalogue Number: AB00102-30.0-BS-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:
Clone:	4-4-20 (enhanced)
Isotype:
Immunogen:	The original antibody was generated by immunizing BALB/c mice with fluorescein I-keyhole limpet hemocyanin (FlI-KLH).
Application:	InVivoA, Ctrl (-)

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody, a humanized, enhanced scFv of the original mouse IgG2a antibody, was constructed by grafting the CDRs of the original antibody onto the humanized 4D5 anti-HER2 antibody framework, and subsequently enhancing it with respect to folding and stability via randomization in the form of DNA shuffling, as well as oligonucleotides encoding mutations at defined positions (Jung et al., 1999; PMID: 10556036). The original antibody was tested against FlI-BSA. The enhanced antibody fragment was tested against FITC-BSA. This antibody can be used as an isotype control in both in vitro and in vivo applications. This antibody is not conjugated to fluorescein.

Short Description

This is scFv was created as part of a panel offering antibodies of the same specificity in different formats (species, isotype, subtype and modified versions) for use as isotype controls.

Specificity

FL; FlI; FITC; Fluorescein isothiocyanate; fluorescein isothiocyanate, isomer 1; Fluorescyl; Resorcinolphthalein; C.I. 45350; solvent yellow 94; D & C yellow no. 7; Angiofluor; Japan yellow 201; Soap yellow; 3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one

AB00102-30.0-BS Datasheet

Price

£3,895.00

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