Anti-CD134 [OX86]
Catalogue Number: AB00110-2.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | OX40; OX-40; Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; CD134; Tnfrsf4; OX-86 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | OX86 |
| Isotype: | IgG2a |
| Immunogen: | This antibody was raised against the recombinant mouse OX40 protein. |
| Application: | ELISA, FC, IF, Agon |
Additional Text
Uniprot ID
P47741
Gene Name
TNFRSF4
Gene ID
22163
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
OX86 antibody, in its original rat IgG1 format, acts as an OX40 agonist in an Fc-dependent manner and has been widely used in studies investigating the role of OX40 in tumor inhibition (Hirschhorn-Cymerman et al., 2009). Studies comparing two recombinant murine Fc versions (IgG1 and IgG2a) elucidated the importance of the FcR engagement in the antitumor activity of this antibody; the study demonstrated that a stronger murine Fc (in an IgG2a version) was more effective in inducing a CT26 syngeneic tumor regression as well as it led to a stronger Treg depletion in the tumor in comparison with a murine IgG1 version (Metzger et al., 2016). In another study two versions of the OX86 antibody (with a fully functional Fc fragment and with an impaired Fc) and showed that Fc-dependent activity was necessary in inducing systemic antitumor activity (Sagiv-Barfi et al., 2018). FC: This antibody was also used in flow cytometry to stain mouse CD4 and CD8 single-positive cells (al-Shamkhani et al., 1996). FC: Blanquiceth et al. (2016) used this antibody in flow cytometry as well to analyse cells coming from mediastinal lymph nodes and lung tissue. IHC: OX86 antibody was utilised in immunohistochemistry staining of gut tissue (Peyer's patches and lamina propria samples) in order to identify OX40-positive cells in colitic mice (Higgins et al., 1999).
Short Description
This is a chimeric antibody created to reduce immunogenicity during in vivo applications.
AB00110-2.3 Datasheet
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