Anti-VAP-1 [TK8-14]
Catalogue Number: AB00168-2.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | TK8-14 |
| Isotype: | IgG2a |
| Immunogen: | The original antibody was generated by immunizing specific pathogen-free BALB/c mice with affinity-purified VAP-1 obtained from tonsil stromal lysate. |
| Application: | ELISA, FC, IF, IP, WB, IHC, Inh, Sq, IAC |
Additional Text
Gene Name
AOC3
Gene ID
8639
Uniprot ID
Q16853
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The original version of this antibody (mouse IgG2a) was generated, and its specificity was characterized by reciprocal immunoprecipitation with another anti-VAP-1 antibody (1B2). In gel analyses, this antibody was shown to react with the dimeric 170-kDa VAP-1 and, under reducing conditions, with the monomeric 90-kDa form of VAP-1. Furthermore, it was used for both immunofluorescence staining and flow cytometry analysis to assess the expression of VAP-1 in rat HEV-like endothelial cell line Ax transfectants (Salmi et al., 1998; PMID: 9605169). The original version of this antibody was biotinylated and characterized via various assays. In an ELISA, it was used to detect serum VAP-1 levels. In a Western blot, it was employed to analyze VAP-1 presence and molecular weight. In immunoaffinity chromatography, it was used to purify VAP-1 from serum samples. In an adhesion assay, it was used to specifically deplete sVAP-1 from serum (Kurkijaervi et al., 1998; PMID: 9686623). This antibody was used in transwell migration assays to study the phenotype of transmigrated monocytes by flow cytometry; it was employed to pretreat primary human hepatic sinusoidal endothelial cells to study its effect on monocyte transmigration. The results showed a significant reduction in monocyte transmigration upon pretreatment, indicating the antibody's role as an inhibitor of VAP-1 function (Zimmermann et al., 2012; DOI: 10.1136/gutjnl-2012-302514b.126). This antibody was used in flow cytometry and immunocytochemistry to detect and characterize human hematopoietic stem cells based on VAP-1 expression. In flow cytometry, this antibody was utilized to stain fresh human bone marrow and cord blood (CB) cells. In an immunocytochemistry assay, it was used to stain FACS-sorted CD34+ CB cells for visualization under a microscope. Furthermore, TK8-14 was utilized in RNAseq analysis to investigate gene expression profiles of VAP-1+ and VAP-1- HSCs (Iftakhar-E-Khuda et al., 2021; PMID: 34719737).
Specificity
Vascular adhesion protein 1; VAP1; Copper amine oxidase; HPAO; Membrane primary amine oxidase; Vascular adhesion protein 1; Amine oxidase [copper-containing] 3; Amine oxidase copper-containing 3; Copper amine oxidase; HPAO; Semicarbazide-sensitive amine oxidase 1; Semicarbazide-sensitive; AOC3; Amine oxidase, copper containing 3; amine oxidase; amine oxidase; Vascular adhesion protein 1; SSAO; copper-containing amine:oxy; EC:1.4.3.21
AB00168-2.0 Datasheet
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