Anti-DNA/RNA G-quadruplex [BG4]
Catalogue Number: AB00174-10.6-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | G4 quadruplex |
| Shipping Condition: | Blue Ice |
| Unit(s): | 500 ug |
| Host name: | Human |
| Clone: | BG4 |
| Isotype: | |
| Immunogen: | DNA/RNA G-quadruplex. |
| Application: | ELISA, IF, EMSA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody has been shown (by ELISA) to bind selectively to G-quadruplex forming DNA and RNA structures. Please that Fab-based versions as well as scFvs, with and without Flag-tag are available, which needs to be considered when replicating published methods. Tsai et al. (2022 et al. PMID: 36440760), report similar activtiy between whole-antibody and scFv-based versions of BG4. Weldon et al. (2016, PMID: 27820800) used Ab00174-1.1 to identify G-quadruplexes in long functional RNAs using an electrophoretic mobility shift assay (EMSA). The mouse IgG1 version of BG4 was shown to have weak cross-reactivity with iMotif structures (2018, PMID: 29686376). iMotif structures can be detected selectively using our anti-iMotif antibody Ab01462.
Short Description
This human antibody Fab-fragment was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. It does not have a FLAG-tag, but a His-tag, which may be used for de
AB00174-10.6-BT Datasheet
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