Anti-DNA/RNA G-quadruplex [BG4]
Catalogue Number: AB00174-24.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | G4 quadruplex |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 500 ug |
| Host name: | Goat |
| Clone: | BG4 |
| Isotype: | IgG |
| Immunogen: | DNA/RNA G-quadruplex. |
| Application: | ELISA, IF, EMSA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody has been shown (by ELISA) to bind selectively to G-quadruplex forming DNA and RNA structures. Please that Fab-based versions as well as scFvs, with and without Flag-tag are available, which needs to be considered when replicating published methods. Tsai et al. (2022 et al. PMID: 36440760), report similar activtiy between whole-antibody and scFv-based versions of BG4. Weldon et al. (2016, PMID: 27820800) used Ab00174-1.1 to identify G-quadruplexes in long functional RNAs using an electrophoretic mobility shift assay (EMSA). The mouse IgG1 version of BG4 was shown to have weak cross-reactivity with iMotif structures (2018, PMID: 29686376). iMotif structures can be detected selectively using our anti-iMotif antibody Ab01462.
Short Description
This chimeric goat antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
AB00174-24.1-BT Datasheet
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