Anti-CD32 [AT130-5]
Catalogue Number: AB00287-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | FcγRIIB; FcgRIIb; CD32B; CDw32; FCG2; FCGR2; FCGR2B; IGFR2; Immunoglobulin G Fc receptor II; Low affinity immunoglobulin gamma Fc region receptor II-b; MGC23887; MGC30032; RP11-5K23.6 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | AT130-5 |
| Isotype: | IgG |
| Immunogen: | The original antibody was generated by immunizing BALB/c FcγRIIB-/- mice with FcγRIIB-rat CD4 domain 3/4 fusion protein. |
| Application: | FC, IF, WB, IHC, Blk, Stim |
Additional Text
Gene Name
FCGR2B
Gene ID
14130
Uniprot ID
P08101
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The original version of this antibody (mouse IgG1) was used in various FACS, Western blot, and immunofluorescence experiments to demonstrate that, for example, interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 mAb (White et al., 2011; PMID: 21742972), and that immunotherapy targeting murine FcγRIIB is limited by mAb consumption and receptor internalization (Williams et al., 2013; PMID: 24026082). It is noted that, unlike the antagonistic anti-CD32 (clone AT128) antibody, which blocks FcγRIIB receptor phosphorylation, this agonistic anti-CD32 (clone AT130-5) has been shown to activate receptor function while blocking immune complex engagement to the inhibitory FcγRs (Vollack et al., 2017; PMID: 28492697). These differential antagonistic/agonistic anti-CD32 mAbs have been used to probe more specifically the role of CD32 in the murine FVIII-specific recall response (Vollack et al., 2017; PMID: 28492697).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB00287-23.0-BT Datasheet
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