Anti-Ly6G [1A8]
Catalogue Number: AB00295-2.0-VXB-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | LGR-1 ; y-6G; GR1; Lymphocyte antigen 6G |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 mg |
| Host name: | Mouse |
| Clone: | 1A8 |
| Isotype: | IgG2a |
| Immunogen: | LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells |
| Application: | FC, Depl |
Additional Text
Gene Name
Ly6g
Uniprot ID
P35461
Gene ID
546644
Antibody Clonality
Recombinant Monoclonal
Storage Note
All VivopureX products are formulated in PBS only without addition of preservatives. To ensure optimal storage and prevent microbial contamination, only open and dispense under sterile conditions. Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022; PMID: 36359801).
Short Description
This is a murinised antibody to reduce immunogenicity in vivo, however with an isotype chosen to promote depletion of targeted cells via ADCC and CDC
AB00295-2.0-VXB Datasheet
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