Anti-Carbonic Anhydrase IX [M75]

Catalogue Number: AB00414-1.1-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	CA9; CAIX; MN/CA IX; endogenous protein p54/58N
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Mouse
Clone:	M75
Isotype:	IgG1
Immunogen:	The original antibody was generated by immunizing BALB/C mice with MaTu-infected HeLa cells.
Application:	ELISA, FC, IF, WB, IHC, Blk, IB, ITC, Crstapy

Overview
Datasheets

Additional Text

Gene ID

768

Gene Name

CA9

Uniprot ID

Q16790

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastorekova et al., 1992; PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Zavada et al., 2000; PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its Kd was determined to be 0.42 µM (Kral et al., 2008; PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al., 2009; PMID: 19538935).

Short Description

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

AB00414-1.1-BT Datasheet

Price

£1,655.00

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