Anti-Carbonic Anhydrase IX [M75]
Catalogue Number: AB00414-3.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CA9; CAIX; MN/CA IX; endogenous protein p54/58N |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | M75 |
| Isotype: | IgG2b |
| Immunogen: | The original antibody was generated by immunizing BALB/C mice with MaTu-infected HeLa cells. |
| Application: | ELISA, FC, IF, WB, IHC, Blk, IB, ITC, Crstapy |
Additional Text
Gene ID
768
Gene Name
CA9
Uniprot ID
Q16790
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastorekova et al., 1992; PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Zavada et al., 2000; PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its Kd was determined to be 0.42 µM (Kral et al., 2008; PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al., 2009; PMID: 19538935).
Short Description
This is a reformatted mouse IgG2b Fc Silent™ antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.
AB00414-3.3 Datasheet
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