Anti-TSY-LeuT [6A10]
Catalogue Number: AB00462-1.6-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Bacterial Leucine Transporter |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | 6A10 |
| Isotype: | |
| Immunogen: | Purified LeuT in detergent. |
| Application: | WB, FSEC |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Uniprot ID
O67854
Application Notes
The antibody binds specifically to LeuT is a bacterial symporter specific for small hydrophobic amino acids such as leucine and alanine, driven by the sodium electrochemical gradient across the membrane. The antibody is specific for the inward-open conformation of LeuT, which required weakening of the Na2 binding site by mutating Thr 354 to Val, and Ser 355 to Ala in TM8, and the mutation of Tyr 268 to Ala in TM6 of the cytoplasmic gate. (TSY_LeuT). The Fab fragment of the antibody was obtained by papain digestion. Due to the high homology of LeuT to human neurotransmitter transporters, its crystal structure can reveal the mechanism of action of many drugs, such as antidepressants.
Short Description
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB00462-1.6 Datasheet
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