Anti-CD4 [OX-68]
Catalogue Number: AB00587-8.1-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Leu3/T4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rat |
| Clone: | OX-68 |
| Isotype: | IgG2b |
| Immunogen: | This antibody was prepared by subcutaneously injecting BALB/c mice with a soluble form of rat CD4, consisting of domains 3 and 4. A boost of a mixture of free sCD4 and sCD4 bound to cells was administered intravenously. |
| Application: | FC, IP, WB, SPR, BndA |
Additional Text
Gene Name
CD4
Gene ID
24932
Uniprot ID
P05540
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody binds to rat CD4. Upon challenge with HIV-1, this antibody was able to significantly inhibit viral entry into HeLa cells expressing a mutant of rat CD4 that binds gp120 (SImon, 1997). Similarly, pre-binding of gp120 resulted in a 50% reduction in binding of this antibody, indicating epitope masking. Analogous effects on viral entry and synctium formation are observed with MAbs against human CD4.
Short Description
This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
AB00587-8.1 Datasheet
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