Anti-Ebola surface glycoprotein [KZ52]

Catalogue Number: AB00690-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	trimeric prefusion Ebola virus glycoprotein; EBOV GP
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Rabbit
Clone:	KZ52
Isotype:	IgG
Immunogen:	This antibody was derived from a human survivor of the 1995 Kikwit outbreak of Ebola.
Application:	IF, IP, NT, Crstion

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody neutralizes Zaire ebolavirus in vitro and offers protection from lethal EBOV challenge in rodent models, but has minimal effects on viral pathogenicity in non-human primates. KZ52 likely neutralizes by preventing rearrangement of the GP2 HR1A/HR1B segments and blocking host membrane insertion of the internal fusion loop. Alternatively, IgG KZ52 may sterically hinder access to the RBS or to a separate binding site of another cellular factor, especially if multiple attachment events are required for entry (Lee et al. 2009). This antibody will detect both live and fixed virus by IF (Maruyama 1999, PMID: 10364354) . It has also been used as a positive control in the development of anti-Ebola polyclonal goat antibodies (Boisen et al. 2015).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB00690-23.0 Datasheet

Price

£390.00

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