Anti-Acetylaminofluorene [K16-16]
Catalogue Number: AB00759-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | 2-AAF; AAF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rabbit |
| Clone: | K16-16 |
| Isotype: | IgG |
| Immunogen: | K16-16 mAb was generated by immnizing BALB/c mice with acetyl aminofluorene-substituted guanosine (Le Guernet al, 1984). RNA derived from this hybridoma clone was then used to construct a phage display library and was selected for by its affinity for AAF-labelled sonicated salmon sperm DNA in an ELISA screen. |
| Application: | SB, PCR, DB, PlqHyb |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
K16-16 was used to construct a recombinant conjugate by fusing PhoAv bacterial phosphatase. The conjugate was used for one-step immunodetection of DNAs and RNAs in dot-blot, colony and plaque hybridization and in Southern/northern blot hybridization. It has also been used to detect PCR products.
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv (IgG1) format, for improved compatibility with existing reagents, assays and techniques.
AB00759-23.0 Datasheet
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