Anti-HBsAg [F124]
Catalogue Number: AB00768-10.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | HBV M protein; Hepatitis B virus surface antigen; Hepatitis B surface antigen; HBV surface antigen; HBV-sAg; Hepatitis B virus M protein; Major surface protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Human |
| Clone: | F124 |
| Isotype: | IgG1 |
| Immunogen: | BALB/c mice were immunized by injection of HBV particles serotype ay, purified from HBsAg-positive patient blood sera. Splenocytes from immunized mice were then fused with the Sp2/0-Ag myeloma cell line. |
| Application: | ELISA, WB, RIA, NT |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
UniProt ID
Q773S4
Application Notes
F124 was used as a solid-phase coating for RIAs detecting HBV particles, resulting in particle immobilisation by binding the pre-S2 epitope of the surface antigen (Budkowska et al, 1986). An scFv-formatted version of F124, comprising the same VH and VL sequences, showed dose-dependent binding to, and competitive blocking of IgG and Fab against, r-HBsAg in an ELISA (Passafiume et al, 1998). Size-exclusion HPLC indicates that, while the scFv monomer shows no tendency to aggregate at low concentrations, equilibrium is achieved at a monomer/dimer ratio of 3:1 at high protein concentrations.
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB00768-10.0-BT Datasheet
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