Anti-disialoganglioside 2 [7A4]
Catalogue Number: AB00770-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | GD2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | 7A4 |
| Isotype: | IgG |
| Immunogen: | 7A4 was generated by hyperimmunizing mice with recombinant gamma-interferon-treated LAN-1 neuroblastoma cells. |
| Application: | ELISA, IF, RIA, IHC, Depl, TLC |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
7A4 is able to lyse tumour cells by complement-mediated cytotoxicity (lytic dose ~10 ng) and can perform in vitro direct binding of labelled mAb to living and glutaraldehyde-fixed cells. Shows strong binding to all neuroblastomas tested in addition to other GD2-expressing cells such as melanomas, small-cell lung carcinomas, rhabdomyosarcomas, Ewings sarcomas, Wilm's tumors and retinoblastomas. TLC analysis can be used to confirm the antibody target antigen. The 7A4 scFv possesses the same specificity as the full-length antibody but slightly weaker binding affinity. The scFv has been used in IF studies (PMID: 9309424).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB00770-23.0-BT Datasheet
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