Anti-Z-DNA/Z-RNA [Z22]

Catalogue Number: AB00783-10.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Z-22; Z 22; Z DNA; ZDNA; Z RNA; ZRNA; Z NA; Z-NA; ZNA
Shipping Condition:	Blue Ice
Storage Condition:	2-8°C
Unit(s):	1 mg
Host name:	Human
Clone:	Z22
Isotype:	IgG1
Immunogen:	Z22 was prepared by immunizing C57BL/6 mice with brominated poly(dG-dC).poly(dG-dC) complexed with methylated bovine serum albumin (BSA), and was selected for by ELISA. Brominated poly(dG-dC).poly(dG-dC) forms a stable Z-DNA helix under physiological salt conditions.
Application:	ELISA, FC, IF, IP, IHC, EMSA, IB, InVitroA, SPR

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

Z22 binding to Z-DNA was evaluated by a competitive solid-phase ELISA (Moeller et al., 1982; PMID: 7118931). The binding affinity of Z22 Fab version engineered from the original mouse IgG2b format to Z-DNA by SPR was measured to have an apparent KD of ~160 nM and could be competed out by soluble brominated d(G-C)15 but not unmodified d(G-C)15 (B-DNA). Z22 scFv binds to the target with a similar affinity to Z22 Fab. Competitive ELISA was used to determine binding specificity. Gel retardation assays were also used to show Z22 binding to DNA-8-MOP adducts. Biotinylated Z22 was used to determine the distribution of Z-DNA in permeabilized, microbead-encapsulated nuclei after adding radioactive streptavidin, and compared to encapsulated permeabilized nuclei stained with DAPI (Wittig et al., 1989; PMID: 2921282). Z22 was shown to cross-react with Z-RNA (Zarling et al., 1990; PMID: 2153833). Z-RNA binding using this product (Ab00783-3.0) was shown using IF (Zhang et al., 2020; PMID: 32200799), and it was also used in FC (Koehler et al., 2021; PMID: 34192517). Furthermore, this product (Ab00783, unspecified isotype) was also used in IHC and IB (Yau et al., 2021; PMID: 34728780), and Electrophoretic Mobility Shift Assay (EMSA; gel mobility assay) (Li et al., 2022; PMID: 35744832).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

AB00783-10.0-BT Datasheet

Price

£1,805.00

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