Anti-Z-DNA/Z-RNA [Z22]

Catalogue Number: AB00783-2.3-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Z-22; Z 22; Z DNA; ZDNA; Z RNA; ZRNA; Z NA; Z-NA; ZNA
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Mouse
Clone:	Z22
Isotype:	IgG2a
Immunogen:	Z22 was prepared by immunizing C57BL/6 mice with brominated poly(dG-dC).poly(dG-dC) complexed with methylated bovine serum albumin (BSA), and was selected for by ELISA. Brominated poly(dG-dC).poly(dG-dC) forms a stable Z-DNA helix under physiological salt conditions.
Application:	ELISA, FC, IF, IP, IHC, EMSA, IB, InVitroA, SPR

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Short Description

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Application Notes

Z22 binding to Z-DNA was evaluated by a competitive solid-phase ELISA (Moeller et al., 1982; PMID: 7118931). The binding affinity of Z22 Fab version engineered from the original mouse IgG2b format to Z-DNA by SPR was measured to have an apparent KD of ~160 nM and could be competed out by soluble brominated d(G-C)15 but not unmodified d(G-C)15 (B-DNA). Z22 scFv binds to the target with a similar affinity to Z22 Fab. Competitive ELISA was used to determine binding specificity. Gel retardation assays were also used to show Z22 binding to DNA-8-MOP adducts. Biotinylated Z22 was used to determine the distribution of Z-DNA in permeabilized, microbead-encapsulated nuclei after adding radioactive streptavidin, and compared to encapsulated permeabilized nuclei stained with DAPI (Wittig et al., 1989; PMID: 2921282). Z22 was shown to cross-react with Z-RNA (Zarling et al., 1990; PMID: 2153833). Z-RNA binding using this product (Ab00783-3.0) was shown using IF (Zhang et al., 2020; PMID: 32200799), and it was also used in FC (Koehler et al., 2021; PMID: 34192517). Furthermore, this product (Ab00783, unspecified isotype) was also used in IHC and IB (Yau et al., 2021; PMID: 34728780), and Electrophoretic Mobility Shift Assay (EMSA; gel mobility assay) (Li et al., 2022; PMID: 35744832). The rabbit IgG version of this antibody (Ab00783-23.0) was successfully used in a dot blot assay at a concentration of 2 µg/ml to detect Z-RNA in total cellular RNA extracted with TRIzol (Bae et al., 2025; PMID: 40425585).

AB00783-2.3-BT Datasheet

Price

£1,825.00

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