Anti-EDAR [EDAR3]
Catalogue Number: AB00784-10.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | ectodysplasin A receptor; Tumor necrosis factor receptor superfamily member EDAR; Anhidrotic ectodysplasin receptor 1; Downless; Ectodermal dysplasia receptor; Ectodysplasin-A receptor |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Human |
| Clone: | EDAR3 |
| Isotype: | IgG1 |
| Immunogen: | EDAR3 was prepared by immunizing female OVE1B mice (with the Edar gene deleted) subcutaneously with human EDAR-Fc and positive hybridoma clones were screened for binding hEDAR by ELISA. |
| Application: | ELISA, WB, SPR, Agon |
Additional Text
Gene Name
EDAR
Gene ID
10913
Uniprot ID
Q9UNE0
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
EDAR3 can be used in surrogate reporter assay in which Fas-sensitive cells were transfected with a construct formed by fusing the ectodomain of mouse or human EDAR to the intracellular domain of Fas. Binding of EDAR3 resulted in the induction of apoptosis in these cells, confirming the agonisitc activity of the antibody. (EC50 (dose required to kill half of EDAR:Fas-expressing Jurkat cells) hEDAR:Fas ~ 100 ng/ml; EC50 mEDAR:Fas ~ 30 ng/ml - both were less active than DEA1-Fc). The agonistic activity was shown to be cross-reactive with EDA-deficient dogs, in which dentition was corrected when the antibody was administered intravenously at 2 and 14 days old. Lacrimation was also improved in the 2 day old dogs. Tail-hair formation was also rescued after administration of EDAR3 to newborn EDA-deficient Tabby mice, with 0.18 mg/kg required for half-maximal tail-hair reversion. EDAR3 can also be used in ELISA and as a Fab fragment (generated by ficin digestion) in SPR experiments. EDAR is recognized by EDAR3 in WB under non-reducing conditions.
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB00784-10.0 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations