Anti-RNA polymerase II [CTD 4H8]

Catalogue Number: AB00832-10.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	RNAPII; DNA-directed RNA polymerase subunit RPB1; RNA polymerase II subunit B1
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	CTD 4H8
Isotype:	IgG1
Immunogen:	Murine antibodies were produced by immunising mice with a peptide consisting of 10 repeats of the RNA polymerase II C-terminal domain sequence YSPTSPS. In each repeat, Ser5 was phosphorylated. Immunised cells were fused with the Sp2/0-Ag14 myeloma cell line to produce a hybridoma.
Application:	ELISA, FACS, FC, IF, IP, ChIP, WB, IHC

Overview
Datasheets

Additional Text

Gene ID

5430

Gene Name

POLR2A

Uniprot ID

P24928

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

This mAb has been used in a variety of methods, including Western blotting of the unphosphorylated (Engelhardt et al, 2005) or Ser5-CTD phosphorylated form (Stock et al, 2007), and in precipitation experiments either simply pulling down RNAPII (Kukalev et al, 2005; Peng et al, 2014) or coupled to massively parallel DNA sequencing (ChIP-seq) of the total RNAPII-associated DNA isolated from human CD4+ cells (Zhang et al, 2012).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB00832-10.0 Datasheet

Price

£390.00

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