Anti-CD29 [12G10]
Catalogue Number: AB00849-10.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Integrin beta-1; Integrin β1 chain; Integrin β1; VLA-β chain; Glycoprotein Iia; GPIIA; Fibronectin receptor subunit beta; ITGB1; VLA-4 subunit beta; FNRB; MDF2; MSK12 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Human |
| Clone: | 12G10 |
| Isotype: | IgG1 |
| Immunogen: | This antibody was raised by immunising BALB/C mice with purified β1 integrins from HT-1080 fibrosarcoma cells |
| Application: | ELISA, FC, WB, IHC, Enhmnt |
Additional Text
Gene ID
3688
Gene Name
ITGB1
Uniprot ID
P05556
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody reacts with β1 integrins in Western blotting (Mould et al, 1995). ELISA analysis has revealed the ability of this antibody to enhance the interaction between β1 integrin and its ligand, fibronectin (Mould et al, 1995). Indeed, activation of integrins by this antibody reduced the induction of osteosarcoma growth and inhibition of NF-κB caused by hERG1 knockdown in osteosarcoma cells (Zeng et al, 2016). This antibody has been used to specifically label ligand-bound cell surface β1 integrins both in immunohistochemical analysis (Izumi et al, 2015; Chao & Kunz, 2009), including in situ proximity ligation (PLA) analysis (Nevo et al, 2010), and in FACS analysis (Brandt et al, 2009).
Short Description
This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB00849-10.3 Datasheet
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