Anti-MHC I [R1-9.6]
Catalogue Number: AB00879-7.4-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | R1.9.6; H-2; MHC-I; MHC Class I |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rat |
| Clone: | R1-9.6 |
| Isotype: | IgG2a |
| Immunogen: | This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas. |
| Application: | IF, IP, RIA |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody binds murine H-2 MHC class I molecules and has been used in both structural and biochemical studies. This antibody has been used in immunoprecipitation analysis (Robinson, 1987) and in immunofluorescence assays for MHC-I surface expression (Karacsonyi et al., 2004) and MHC-I endocytosis (Knorr et al., 2009). This antibody has also been employed to induce clustering of cell surface MHC-I in co-patching assays (Knorr et al., 2009). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells and assess its strain specificity (Koch et al., 1983; PMID: 6192569).
Short Description
This chimeric rat antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.
AB00879-7.4-BT Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations