Anti-TCR [H57-597]
Catalogue Number: AB00903-22.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | TCR-beta; T cell receptor; T-cell receptor; T cell receptor beta chain; TCRB; TRB; TCB1; Ti |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Armenian Hamster |
| Clone: | H57-597 |
| Isotype: | IgG |
| Immunogen: | Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas. |
| Application: | FC, IP, IHC, Crstion |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Uniprot ID
P01852
Application Notes
In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2. H57-597 has also been used to construct a Fab fragment which has been complexed with deglycosylated TCRs for their crystallisation for structure determination (Liu 1996). Flow cytometry is another application of this antibody, where H57-597 has been used to analyse TCR-beta expression in different T cell populations (Tsukumo 2006).
AB00903-22.0 Datasheet
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