Anti-HER2 [C7b]
Catalogue Number: AB00924-1.9-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 1 mg |
| Host name: | Mouse |
| Clone: | C7b |
| Isotype: | IgG1 |
| Immunogen: | A llama was immunized with the HER2-expressing ovarian cancer cell line SKOv3 and a sdAb phage display library was built and used to perform biopannings. The first round of selection was performed on a purified recombinant HER2 ectodomain fused to a human Fc portion (HER2-Fc) followed by a second round on an HER2-positive SKBr3 cell line. After selection, 188 clones were screened and 92 clones were found to be positive by ELISA on HER2 positive cells. Twenty clones were analyzed by sequencing, and all of them corresponded to a unique clone named C7b. |
| Application: | ELISA, FC, Cell Assay, WB, IHC |
Additional Text
Gene ID
2064
Gene Name
ERBB2
Uniprot ID
P04626
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Specificity
NEU; HER-2; HER-2/neu; ERBB2; CD340; MLN 19; NGL; TKR1; erb-b2 receptor tyrosine kinase 2; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
The fine specificity of this antibody was determined by ELISA. The ELISA was preformed using the VHH version of this antibody on HER2 fused to an fc fragment. Furthermore the ability of the antibody to bind to cells expressing HER2 was assessed. This was done by flow cytometry on cells overexpressing HER2 using the VHH version of this antibody (Even-Desrumeaux et al, 2012; pmid:22772166). To assess the reactivity of this antibody, a dot blot was preformed on a ErbB2/HER2 Fc Chimera Protein using the VHH version of the antibody. Further, both a cytotoxicity assay and an internalization assay were preformed using this antibody bound to a model therapeutic nanoparticle (Vorotnikov et al, 2020; pmid:33107540). C7b has also been used to enrich breast cancer cells (SKBr3) from a large excess of human peripheral blood mononuclear cells (Even-Desrumeaux 2012).
Short Description
This chimeric mouse antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
AB00924-1.9-BT Datasheet
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