Anti-Sigma1 [5C6]
Catalogue Number: AB00926-2.3-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Outer capsid protein sigma1; sigma1 protein; Reovirus strain T1L sigma 1; strain T1L S1; T1L S1; type 1 haemagglutinin; type 1 HA; T1L HA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | 5C6 |
| Isotype: | IgG2a |
| Immunogen: | Murine antibodies were produced by immunising CAF1 mice with reovirus strain T3D, with subsequent immunisation boosts using UV-inactivated strain T1L reovirus. Immunised mice produced antibodies recognising both T3D and T1L. Splenocytes were obtained from immunised mice and fused with the P3Ag8.653 myeloma cell line to generate hybridomas. |
| Application: | FC, IF, IP, NT, Crstion |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Uniprot ID
P04506
Application Notes
5C6 was shown recently to block reoviral infection of L929 mouse fibroblasts in a dose-dependent manner, using immunofluorescence staining (Dietrich et al, 2017). Fibroblasts were incubated with virus-antibody mixtures, and immunofluorescence staining of the cells enabled detection of infectivity. 5C6 is highly specific for the T1L reoviral strain, as opposed to the T3D strain which was also tested. 5C6 was also used to immunoprecipitate reoviral particles.
AB00926-2.3 Datasheet
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