Anti-TIGIT [4D4]
Catalogue Number: AB00973-1.69-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | 4D4 |
| Isotype: | |
| Immunogen: | Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants. |
| Application: | FC, Agon |
Additional Text
Uniprot ID
P86176
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity
VSIG9; T Cell Immunoreceptor With Ig And ITIM Domains; V-Set And Immunoglobulin Domain Containing Protein 9; V-Set And Transmembrane Domain Containing Protein 3; VSTM3; T Cell Immunoreceptor With Ig And ITIM Domains; Washington University Cell Adhesion Molecule; WUCAM
Application Notes
4D4 is an agonistic anti-TIGIT antibody, and addition of plate bound 4D4 to anti-CD3/anti-CD28 stimulated T cells was shown to inhibit their proliferation (Joller 2011). Further, 4D4 has been used in order to identify genes that are downstream of TIGIT signaling in Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue (Liu 2016).
Short Description
This is a bispecific anti-mouse antibody created by fusing anti-PDL-1 VHH domains to the C-terminus of the heavy chains) of the anti-mouse TIGIT antibody clone 4D4 in a mouse IgG1 D265 format. The mouse IgG1 D265 a format is an Fc-silenced format, prevent
AB00973-1.69 Datasheet
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