Anti-CD80 [RM80]
Catalogue Number: AB01028-3.3-VXX-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | B7-1; Activation B7-1 antigen; B7; B7 protein; B7-1 protein; B7.1; Cd28l; CD28LG; CD28LG1; LAB7; Ly-53; Ly53; MIC17; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 mg |
| Host name: | Mouse |
| Clone: | RM80 |
| Isotype: | IgG2b |
| Immunogen: | This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells. |
| Application: | FC, WB, IHC, FA, Blk |
Additional Text
Gene Name
CD80
Gene ID
12519
Uniprot ID
Q00609
Antibody Clonality
Recombinant Monoclonal
Storage Note
All VivopureX products are formulated in PBS only without addition of preservatives. To ensure optimal storage and prevent microbial contamination, only open and dispense under sterile conditions.
Application Notes
This antibody has been used in multiple FACS experiments for various immunological applications, such as to highlight the robustness of the T cell priming process by activated Nlrc5-deficient dendritic cells (Rota et al, 2016), to demonstrate that allogeneic IgG combined with dendritic cell stimuli could induce anti-tumor T cell immunity (Carmi et al, 2015), to confirm the role of aquaporin-9-expressing neutrophils for the development of contact hypersensitivity (Moniaga et al, 2015), and to support the therapeutic potential of targeting B-cell Syk signaling in chronic graft-versus-host disease (Flynn et al, 2015). This antibody has also been used in Western Blot to study the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al, 2015), as well as in in vitro blocking experiments to confirm the role of CD28 signals for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) γδ T cell subsets (Ribot et al, 2012). Furthermore, this antibody has been used, together with the anti-CD86 antibody, in numerous in vivo studies, such as in a NZB/W F1 mouse lupus model to clarify the role of CD80 and CD86 in murine Ab-mediated autoimmunity (Nakajima et al, 1995), in graft bearing recipient BALB/c mice to evaluate the roles of CD80 and CD86 costimulatory molecules in corneal allograft rejection (Kagaya et al, 2002), and in transgenic BALB/c mice to demonstrate the critical contribution of CD80 and CD86 in anterior chamber-associated immune deviation (Tsukahara et al, 2005).
Short Description
This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
AB01028-3.3-VXX Datasheet
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