Anti-Tim-4 [5G3]
Catalogue Number: AB01058-23.0-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rabbit |
| Clone: | 5G3 |
| Isotype: | IgG |
| Immunogen: | This antibody was generated by immunizing a female Lewis rat with Tim-4-Ig fusion protein. |
| Application: | ELISA, FACS, FC, IP, InVivoA |
Additional Text
Gene Name
TIMD4
Gene ID
276891
Purification
Purified
Uniprot ID
Q6U7R4
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity
TIMD4; SMUCKLER; SMUCKLER, TIM4; Spleen, mucin-containing, knockout of lymphotoxin protein; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIM-4; TIMD-4
Application Notes
The original version of this antibody (rat IgG1) specifically bound murine Tim-4 protein, as shown in ELISA and FC assays. The antibody was also used to immunoprecipitate Tim-4 protein from lysates of CHO-Tim-4 as part of WB assays (Rodriguez-Manzanet et al., 2008; PMID: 18354194). This antibody can block apoptotic body (AB) recognition by CD11b+ peritoneal macrophages and partially inhibit AB uptake by peritoneal B-1 cells (Rodriguez-Manzanet et al., 2010; PMID: 20368430). treatment with this antibody alone did not significantly affect tumor burden or survival, but it enhanced the protective effect of anti-PD-1 blockade. The combined treatment resulted in an increase in total CD8+ and CD8+ CD39+ T cells in the peritoneal cavity. RNA sequencing of sorted Tim-4+ macrophages revealed genes differentially expressed between animals treated with anti-PD-1 alone and those treated with both anti-PD-1 and this antibody, indicating that it alters the gene expression profile of Tim-4+ macrophages. Notably, it reduced the expression of genes associated with immunosuppression (Ptgs2, Tgfb2, and Tdo2) while increasing the expression of genes associated with macrophage activation (Stab1 and Mrc1). This antibody was used as part of single-cell RNA sequencing and T-cell receptor sequencing analyses. This antibody was used for FC staining in the form of flow sorting, co-culture experiments, and flow cytometric analysis to investigate various aspects of Tim-4 function, including its role in the sequestration of cytotoxic CD8+ T cells, the impairment of CD8+ T cell proliferation by cavity-resident macrophages, and the assessment of Tim-4-mediated suppression of CD8+ T cells both in vitro and in vivo. For example, it was used for FC staining in the form of annexin V staining to detect phosphatidylserine (PS) exposure on the surface of CD8+ T cells (Chow et al., 2021; PMID: 34115989).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB01058-23.0 Datasheet
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