Anti-Sonic Hedgehog [5E1]

Catalogue Number: AB01175-23.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	shh; sonic hh; Q15465
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Rabbit
Clone:	5E1
Isotype:	IgG
Immunogen:	This antibody was raised by immunizing mice with purified rat SHH amino-terminal domain protein.
Application:	ELISA, WB, IHC, Blk, Crstion

Overview
Datasheets

Additional Text

Gene Name

SHH

UniProt ID

Q63673

Purification

Purified

Gene ID

29499

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

The binding specificity of this antibody for the amino-terminal fragment of SHH (SHH-N) has been confirmed in ELISA and Western blot analysis (Ericson et al, 1996).This antibody has been used to detect SHH in IHC analysis (Ericson et al, 1996).This antibody is able to inhibit hedgehog signalling, as it blocks the binding of all three mammalian Hh ligands to Ptc1 with low nanomolar affinity (Bosanac et al, 2009). In vivo, this inhibition is responsible for the observed growth retardation of HT55 an HT-29 xenografts in mice tumour models (Yauch et al, 2008). A Fab fragment derived from this antibody has been used in X ray crystallography analysis, in complex with human SHH (Maun, 2010).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB01175-23.0-BT Datasheet

Price

£1,655.00

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