Anti-IdeS-cleaved hinge of IgG1 [C2095]

Catalogue Number: AB01182-10.3-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	IdeS-cleaved hinge of IgG4
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Human
Clone:	C2095
Isotype:	IgG1
Immunogen:	This antibody was raised by immunising rabbit with a KLH-conjugated peptide (CTAPPAPAPELLG).
Application:	ELISA, Depl, ADCC

Overview
Datasheets

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

ELISA demonstrated C2095 has robust binding to IdeS-generated F(ab')2 fragments of human IgG1 and human IgG4 but does not bind IdeS-generated F(ab')2 fragments of human IgG2. It also does not bind the intact IgG1, IgG2 or IgG4 counterparts (Brezski et al, 2014). ADCC and CDC assays showed C2095 is capable of restoring antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity against WIL2-S cells to the otherwise inactive anti-CD20 IgG1 F(ab')2 fragment. It also reinstated ADCC against MDA-MB-231 cells to an anti-CD142 IgG1 F(ab')2 fragment. This antibody was also capable of eliciting both CDC and ADCC to IgG4 F(ab')2 fragments (Brezski et al, 2014).

Short Description

This chimeric human antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques.

AB01182-10.3-BT Datasheet

Price

£1,655.00

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