Anti-FedF [NbFedF9]
Catalogue Number: AB01198-34.11-BS-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | F18 fimbrial adhesin AC; F18 fimbrial adhesin |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Camelid |
| Clone: | NbFedF9 |
| Isotype: | |
| Immunogen: | A Llama was immunised 6 times with purified FedF residues 15-165 over a period of 6 weeks. Lymphocytes were extracted and used to prepare cDNA which was used as a template fthe VHH. These were then transfected into E. coli and the antibodies selected by phage display. This was then subjected to ELISA to confirm the specificity of the antibodies. |
| Application: | ELISA, Blk, SPR |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
UniProt ID
Q47212
Application Notes
It has been shown that this antibody can inhibit the binding of FedF to piglent enterocytes. The structure of this antibody binding to FedF has been resolved to a resolution of 1.5A. This shows that the mechanism of inhibition of FedF binding to epithelial cell surfaces is by competing at the carbohydrate binding site. Both MST and SPR have been used to analyse binding affinities (Moones et al, 2014).
AB01198-34.11-BS Datasheet
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