Anti-Protein C [HPC-4]

Catalogue Number: AB01200-23.0-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	Autoprothrombin IIA; C-tag; Protein C tag; Protein C-tag; HPC4; Vitamin K-dependent protein C; Anticoagulant protein C; Blood coagulation factor XIV; PROC
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Rabbit
Clone:	HPC-4
Isotype:	IgG
Immunogen:	This antibody was raised by immunising mice with human protein C.
Application:	AP, ELISA, Blk

Overview
Datasheets

Additional Text

Gene Name

PROC

Uniprot ID

P04070

Gene ID

5624

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

HPC-4 binds human protein C only in the presence of Ca2+; it binds to the reduced carboxymethylated protein C heavy chain, and it blocks protein C activation but does not bind or inactivate protein C or activated protein C bound to its plasma inhibitor. Furthermore, it is an anti-epitope tag antibody, as it recognizes the EDQVDPRLIDGK sequence (Taylor Jr et al., 1987; 3102560). It has been shown to work in ELISAs to determine expression levels (Velander et al., 1992; PMID: 1465430). HPC-4 has been shown to be effective at purifying proteins tagged with its epitope (Rezaie et al., 1992; PMID: 1283093). It has also been used to block the activation of Protein C by the thrombin-thrombomodulin complex (Stearns et al., 1988; PMID: 2447082) and was used for the same purpose in Baboons to lower the threshold of sepsis (Taylor Jr et al., 1987; PMID: 3102560). HPC-4 has been used in many other studies to observe the function of Protein C by knocking out its function in a Ca2+-dependent manner, purification, and others (Tanaka et al., 2006; PMID: 16398653) (Bae et al., 2007; PMID: 17823308).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB01200-23.0 Datasheet

Price

£390.00

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