Anti-MOG [M3-24]
Catalogue Number: AB01207-6.1-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Myelin oligodendrocyte glycoprotein; rMOG |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Rat |
| Clone: | M3-24 |
| Isotype: | IgG1 |
| Immunogen: | Callithrix jacchus marmosets were used, and experimental allergic encephalomyelitis was induced by the injection of rat MOG (aa1-125) into the marmosettes. The rMOG was expressed in E. coli and purified to homogeneity. The animals were killed 4-70 days after the onset of symptoms of EAE. Bone marrow and spleen cells were obtained from an immunized C. jacchus, the RNA extracted with Trizol reagent and rtPCR used to generate cloning inserts containing Fab portions of IgGk. Phage display was then used to select for the MOG-reactive Fab fragments using the pCOMB3H phage display vector, and binding confirmed using an ELISA. |
| Application: | ELISA, IHC, Blk |
Additional Text
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody has been proposed for the diagnosis and prognosis of multiple sclerosis (MS) or allergic encephalomyelitis (EAE) by using competition assays to determine if there are autoantibodies present in an individual. It has also been proposed that it could be used to predict the severity of the MS or EAE by detecting the proportion of conformation-specific autoantibodies to linear epitope autoantibodies. This antibody has been used in ELISAs and competition assays to characterise its epitope in relation to other antibodies generated against MOG. It was originally generated and tested as a Fab fragment (vod Budingen et al, 2002). Whilst this has been shown to not bind to hMOG, it has been shown to compete with IgG from MS patients for binding to rMOG (Lalive et al, 2006). Analysis has also been done on the amino acid sequence of this antibody (von Budingen et al, 2006).
Short Description
This chimeric rat antibody was made using the variable domain sequences of the original Fab format, for improved compatibility with existing reagents, assays and techniques.
Uniprot ID
Q63349
AB01207-6.1 Datasheet
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