Anti-Transferrin receptor [RI7 217.1.3]
Catalogue Number: AB01232-7.1-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD71; Transferrin receptor protein 1; TR; ; TfR1; Trfr; R17 217.1.3; RI7-217 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rat |
| Clone: | RI7 217.1.3 |
| Isotype: | IgG2a |
| Immunogen: | The antibody was obtained from a fusion of the murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6. |
| Application: | FC, Depl |
Additional Text
Gene Name
TFRC
Gene ID
22042
Uniprot ID
Q62351
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody was generated and used in a flow cytometry assay to identify murine hematopoietic progenitor cells expressing the transferrin receptor (Lesley et al., 1984; PMID: 6319033). The original format of this antibody was tested and found to moderately reduce cell growth. A Fab version of this antibody was generated, its binding was tested, and it was compared to its full antibody counterpart. In contrast to the original antibody format, the Fab fragment did not cause receptor down-regulation or accelerated degradation. Both the Fab fragment and the full antibody were successfully used in an IF staining assay (Lesley et al., 1989; PMID: 2653853). The original fotmat of this antibody was demonstrated to undergo receptor-mediated transcytosis across the blood-brain barrier (BBB), as confirmed by capillary depletion studies, and to effectively transport into the mouse brain in vivo (Lee et al., 2000; PMID: 10688622). Injection of this antibody into mice resulted in CD71+ erythroid splenocyte cell depletion (Torow et al., 2015; PMID: 26195040).
AB01232-7.1-BT Datasheet
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