Anti-Influenza A nucleoprotein [aNP-VHH1]

Catalogue Number: AB01243-10.9-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	IAV NP; Influenza A virus nucleoprotein; Influenza A NP; Protein N; αNP-VHH1
Shipping Condition:	Blue Ice
Unit(s):	200 ug
Host name:	Human
Clone:	aNP-VHH1
Isotype:	IgG1
Immunogen:	The original nanobody was generated by subjecting alpacas to five rounds of immunization with alcohol-fixed influenza virus PR8. The generated phage library underwent three separate methods of panning to isolate influenza virus-specific VHHs.
Application:	FC, IF, IP, EM, WB, NT, Crstion

Overview
Datasheets

Additional Text

Gene Name

Uniprot ID

P03466

Purification

Purified

Gene ID

956531

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.

Application Notes

The original version of this antibody (alpaca VHH) was confirmed to target NP antigens in an immunoprecipitation assay. The specificity of this antibody's binding has been validated using both a His tag and mCherry attachment. Furthermore, X-ray crystallography revealed its binding with IVA NP, where it binds to the NP body domain. Mini-genome assays have shown that this antibody not only interferes with Influenza A replication but also prevents the nuclear import of RNPs. Additionally, the antibody has been utilized for the immunoprecipitation of IVA NP and in Western blotting, further confirming its inhibitory effect on the nuclear localization of its target ribonuclear protein, disrupting the virus's function (Ashour et al., 2015; PMID: 25540369). Flow cytometry, in conjunction with negative-stain EM, demonstrated the interference of influenza A vRNP nuclear import by the VHH version of this antibody. This interference occurs through the masking of the nuclear localization sequence of NPs, proposing a model for the inhibition of vRNP nuclear import (Hanke et al., 2016; PMID: 27965447).

Short Description

This chimeric human antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

AB01243-10.9 Datasheet

Price

£390.00

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