Anti-hINSR [IR 25-49]

Catalogue Number: AB01278-23.0-BT-ABA

Manufacturer:	Vector Laboratories, Inc (ABA)
Type:	Recombinant Monoclonal
Alias:	CD220; human insulin receptor; insulin receptor; HHF5; IR; 25 49
Shipping Condition:	Blue Ice
Unit(s):	1 mg
Host name:	Rabbit
Clone:	IR 25-49
Isotype:	IgG
Immunogen:	This antibody was raised by immunising BALB/c mice with purified human placental insulin receptor.
Application:	IP, Blk, Stim

Overview
Datasheets

Additional Text

Gene Name

INSR

Uniprot ID

P06213

Gene ID

3643

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This antibody has been used in immunoprecipitation assays, with radiolabelled human insulin receptor (hINSR) and hINSR-radiolabelled insulin complexes (Soos et al, 1986), and a dimeric soluble hINSR fragment containing the first four domains of the receptor (amino acids 1-593) (Surinya et al, 2002). It has also been used in immunoblot analysis with purified hINSR (Soos et al, 1986), revealing that it binds to the receptor's alpha subunit. This antibody has been shown to cause >80% inhibition of insulin binding to its receptor, in assays with IM-9 cells (Soos et al, 1986), human adipocytes (Taylor et al, 1987) and NIH 3T3 fibroblasts transfected with hINSR cDNA (Soos et al, 1989). Additionally, this antibody has been shown to mimic the metabolic effects of insulin in serum-starved cells, stimulating 2-deoxyglucose uptake to 90% of the maximal insulin effect at 100nM, and thymidine incorporation to 30% of the maximal insulin effect (Soos et al, 1989). This insulin-mimetic antibody has also been shown to stimulate receptor kinase activity and receptor tyrosine phosphorylation in intact cells (Steele-Perkins et al, 1990; Brindle et al, 1990).

Short Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

AB01278-23.0-BT Datasheet

Price

£1,655.00

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