Anti-LAG-3 [C9B7W]
Catalogue Number: AB01295-2.3-VXX-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | CD223; LAG3; Lymphocyte activation gene 3 protein |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 mg |
| Host name: | Mouse |
| Clone: | C9B7W |
| Isotype: | IgG2a |
| Immunogen: | This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein. |
| Application: | FC, IP, Blk |
Additional Text
Gene Name
LAG3
Uniprot ID
Q61790
Gene ID
16768
Antibody Clonality
Recombinant Monoclonal
Storage Note
All VivopureX products are formulated in PBS only without addition of preservatives. To ensure optimal storage and prevent microbial contamination, only open and dispense under sterile conditions.
Application Notes
This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild-type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen-induced IL-2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015).
Short Description
This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB01295-2.3-VXX Datasheet
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