Anti-Integrin beta-3 [Y2/51]
Catalogue Number: AB01334-23.0-BT-ABA
| Manufacturer: | Vector Laboratories, Inc (ABA) |
| Type: | Recombinant Monoclonal |
| Alias: | Integrin b3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61; GP3A; INGRB3; ITG B3; ITGB3; Integrin β3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 1 mg |
| Host name: | Rabbit |
| Clone: | Y2/51 |
| Isotype: | IgG |
| Immunogen: | This antibody was raised by immunising Balb/c mice with PHA-stimulated peripheral blood cells and subsequent fusing spleen cells and NSl myeloma cells. |
| Application: | FACS, FC, IF, WB, IHC |
Additional Text
Gene Name
ITGB3
Gene ID
3690
Uniprot ID
P05106
Purification
Purified
Antibody Clonality
Recombinant Monoclonal
Storage Note
Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Application Notes
This antibody has been extensively used as a marker of megakaryocytes. It is very effective in the immunohistochemical detection of platelets and megakaryocytes in formalin-fixed tissue samples as well as via Western blot and was also recommended for the identification of blood clots in blood vessels (Gatter et al., 1988). Y2/51 antibody has been also found useful in characterisation of other cells and tissues. For instance, one group used it (in concert with other antibodies) to compare immunohistochemically fibroblasts from normal and ruptured human anterior cruciate ligaments for tissue engineering purposes (Brune et al., 2007). Finally, another group performed immunofluorescence staining of HCT15 cells with this antibody and noted increased Integrin beta-3 expression in butyrate-preselected cells in a study on the acquisition of butyrate-resistance by colon cancer cells (Serpa et al., 2010).
Short Description
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
AB01334-23.0-BT Datasheet
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