Anti-Biotin [BK-1/39]

Catalogue Number: AB01346-24.1-BT-ABA

Manufacturer:Vector Laboratories, Inc (ABA)
Type:Recombinant Monoclonal
Alias:vitamin B7; vitamin H; coenzyme R
Shipping Condition:Blue Ice
Unit(s): 1 mg
Host name: Goat
Clone: BK-1/39
Isotype: IgG
Immunogen: This antibody was raised by immunising mice with biotin conjugated to keyhole limpet haemocyanin.
Application: FC, IP, ChIP, IHC

Additional Text

Purification

Purified

Antibody Clonality

Recombinant Monoclonal

Storage Note

Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.

Application Notes

This monoclonal antibody can be used to detect biotinylated proteins and antibodies, particularly if signal amplification is desired. For instance, PE-conjugated BK-1/39 antibody was used to target biotinylated anti-Ig beta antibody in a flow cytometric analysis (Levit-Zerdoun et al., 2016). This antibody was also successfully utilised in the chromatin immunoprecipitation (ChIP) of biotinylated sense or antisense strands of the saRNA duplex (Wu et al., 2016). BK-1/39 was used in in situ hybridisation as well; it detected biotinylated RNA probe complementary to the Epstein-Barr virus genome (de Lima et al., 2012).

Short Description

This chimeric goat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.